The difference BL21 (DE3) and MKK3 expressed in either Rosetta or BL21 (DE3) with added chaperones, even though the yields of total protein obtained from expression was lower or comparable to other strains. Notable in MKK3, despite the presence of aggregated forms of the protein in BL21 with and without chaperones. A one-way ANOVA with Dunnett's multiple comparison test was employed to evaluate differences in expression levels of recombinant protein between Evo21(DE3) and other expression hosts. P values BL21 (DE3) showed the highest level of expression in inclusion bodies followed by Rosetta-gami (DE3) and Shuffle T7. Changes of expression conditions were insufficient for soluble expression of reteplase in SHuffle T7 as a genetically engineered host for production of disulfide bonded proteins. What is the difference between BL21 (DE3) pLysS and BL21 (DE3) pLysE? There are two cell lines that contain the T7 lysozyme; these are called BL21 (DE3) pLysS and BL21 (DE3) pLysE. The following table summarizes some of the key features of the two cell lines. In this work, four bacterial strains, BL21 (DE3), BL21 (DE3) pLysS, Rosetta, and Arctic Express, were chosen for parallel expression trials along with BL21 (DE3) complemented with folding chaperones DnaJ/K and GroEL/ES to compare their performance in producing soluble and active human kinases. BL21 (DE3) Expression from T7 promoter, tight regulation: BL21 (DE3) pLysS: Expression from T7 promoter with codon bias correction: BL21 codon plus, Rosetta: Improved disulphide bond formation: Origami: Fast cloning (due to quick cell growth) Mach1 BL21 (DE3) produced the highest amount of ADI protein, followed by Rosetta (DE3). The following activity assay showed that ADI from BL21 (DE3) and Rosetta (DE3) had the most activity. HnARd.

difference between rosetta and bl21